Naheed Mojgani
Natural Biotechnology Company, Karaj- IR Iran
Title: Antibiotic Resistance in Lactic Acid Bacteria Isolated from Commercial Probiotic Products in Iran
Biography
Biography: Naheed Mojgani
Abstract
A major public health issue worldwide is the emergence of antibiotic resistance and in this regard probiotic Lactic Acid Bacteria (LAB) harboring antibiotic resistance genes are of great concern for consumers and health care providers. Hence, it is of great importance to distinguish intrinsic or acquired character of antibiotic resistance especially in Lactic Acid bacteria used in food chains. In this study we assessed the antibiotic resistance patterns of a LAB strains isolated from commercially available probiotic supplements available in Iranian market and characterized the genetic determinants responsible for the resistances. Forty five LAB isolated from 24 commercial probiotic products (supplements) were identified to species level by microbiological, biochemical and 16S rRNA sequencing method. The antibiotic resistance pattern of the selected isolates was tested by disc diffusion and MIC against 10 antibiotic groups (Carbapenems, Cephalosporins, Glycopeptides, Lincosamdes, Macrolides, Penicillins, Quinolones, Sulfonamides, Tetracyclines, etc). Resistant genes in the strains were examined by PCR analysis and their transferability to other bacteria was evaluated by conjugation studies. Distribution of resistance was found in different species. All tested isolates were susceptible to chloramphenicol, tetracycline, ampicillin, cephalothin, and clindamycin. In contrast, all isolates were resistant to vancomycin, rifampicin, kanamycin, ciprofloxacin, trimethoprim/sulphamethoxazole, and metronidazole. Additionally, a number of strains were resistant to trimethoprim/sulphamethoxazole, and cefepime. Intrinsic aminoglycosides ,quinolones ,sulfonamides and metronidazole resistance were observed in all strains . The vanX ,vanA and tet(M) genes were detected on plasmid DNA of certain LAB isolates. For chloramphenicol,β-lactames no resistance was detected. CONCLUSION: Antibiotic resistance is present in different species of probiotic strains, which poses a threat to food safety. Evaluation of the safety of lactic acid bacteria for human consumption should be guided by established criteria, guidelines and regulations.