Bhukya Prakash
ICAR – Directorate of Poultry Research, India
Title: Production of Selenium Enriched Saccharomyces Cerevisiae using Yeast Extract Peptone Dextrose Broth
Biography
Biography: Bhukya Prakash
Abstract
Dietary supplementation of organic Selenium (Se) through Se enriched yeast is common practice. The Saccharomyces cerevisiae (SC) is recognized well for its ability to accumulate and convert inorganic Se to organic Se. However, the concentration of the sodium selenite (inorganic) determines the growth of SC, which is the critical in producing the organic Se using SC. Therefore, the present experiment was carried out to optimize the concentration of sodium selenite to produce the maximum yeast cell growth. For that purpose, SC (strain 101) was subjected to various concentrations of sodium selenite (0, 10, 20, 30, 40, 50, 60, 80, 100 and 150 mg/L) in yeast extract peptone dextrose (YEPD) broth containing 2% sugar. The SC inoculated broth was maintained in the shaking incubator at 31ºC for 48 h. The broth was then subjected for serial dilution and enumerated using the spread plate (incubated at 48 h at 31ºC) method in the YEPD agar. It was recorded that the growth of SC was decreased 25% and 50% in 10 and 20 mg/L broth, respectively compared to control. However, there is insignificant difference for the colony forming units between the 20 and 30 mg/L levels. Further, Se in the broth of above 50 mg/L considerably reduced the growth of SC. Similarly, the morphological observation revealed that the cells size of SC was decreased and gradual increase in the number of budding was evident. Further above 50 mg/L concentration the rupturing of the cell wall of the SC was observed. Therefore, it has been concluded that the maximum cell growth can be obtained at 10 to 20 ppm concentration of inorganic Se in order to produce the more of the Se enriched SC.